New PDF release: Analytical Ultracentrifugation VI

By W. Mächtle, M. D. Lechner (auth.), W. Borchard, A. Straatmann (eds.)

ISBN-10: 354042489X

ISBN-13: 9783540424895

ISBN-10: 3540446729

ISBN-13: 9783540446729

This quantity contains 20 contributions of the twelfth assembly on Analytical Ultracentrifugation from March 1-2, 2001 in Duisburg, Germany. numerous fields of ultracentrifugation are coated relating learn difficulties in biochemistry, biophysical chemistry and macromolecular chemistry in addition to interacting platforms. New investigations in regards to the sedimentation thought are awarded. The part transition of gels is handled, as is the sedimentation-diffusion equilibrium of gels. One part includes the hydrodynamics of biopolymers.

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13, 31]), combined with a recently introduced linear least-squares method for the calculation of an apparent sedimentation coefficient distribution, was applied. The latter method is based on boundary modelling by a superposition of sedimentation profiles of ideal nondiffusing particles [18]. Our approach certainly lacks the high-throughput capability of the noncommercial systems and lacks the capability of permitting the high-resolution analysis of extremely broad size distributions in a single run, as offered by the ‘‘gravitational sweep method’’ [17, 30].

14 ml/g or 19%. We have also tried to calculate the partial specific volume of the PEG derivative in aqueous solutions using established methods [27, 28]. 2% higher than the experimental results (the same deviation was observed Table 1 Specific volumes of the poly(ethylene glycol) derivative in different solvents and solvent mixtures, as determined by digital densimetry and the buoyant density method. The density, q0, and the dielectric constant, e, of the solvents, at 20 °C, are taken from Refs. 743 28 with unmodified PEG [27]).

1a) was obtained by modifying a commercial precursor (Fig. 3 mg 1-pyrene isothiocyanate (Molecular Probes) in 2 ml DMF was added, and the mixture was stirred for 48 h at 40 °C. After evaporation of the solvent, the compound was redissolved in dichlormethane and purified by size-exclusion chromatography on Bio Beads SX1 (Bio Rad; 70 · 5 cm) in the same solvent. According to matrix-assisted laser desorption ionization time of flight mass spectroscopy using a Voyager DE Pro apparatus (Perceptive Biosystems) with a-cyano cinnamic acid/tetrahydrofuran (THF) as a matrix, the purified compound was characterized by Mn ¼ 3,637 g/mol and Mw ¼ 3,679 g/mol.

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Analytical Ultracentrifugation VI by W. Mächtle, M. D. Lechner (auth.), W. Borchard, A. Straatmann (eds.)


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