By W. Mächtle, M. D. Lechner (auth.), W. Borchard, A. Straatmann (eds.)
This quantity contains 20 contributions of the twelfth assembly on Analytical Ultracentrifugation from March 1-2, 2001 in Duisburg, Germany. numerous fields of ultracentrifugation are coated relating learn difficulties in biochemistry, biophysical chemistry and macromolecular chemistry in addition to interacting platforms. New investigations in regards to the sedimentation thought are awarded. The part transition of gels is handled, as is the sedimentation-diffusion equilibrium of gels. One part includes the hydrodynamics of biopolymers.
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Extra info for Analytical Ultracentrifugation VI
13, 31]), combined with a recently introduced linear least-squares method for the calculation of an apparent sedimentation coeﬃcient distribution, was applied. The latter method is based on boundary modelling by a superposition of sedimentation proﬁles of ideal nondiﬀusing particles . Our approach certainly lacks the high-throughput capability of the noncommercial systems and lacks the capability of permitting the high-resolution analysis of extremely broad size distributions in a single run, as oﬀered by the ‘‘gravitational sweep method’’ [17, 30].
14 ml/g or 19%. We have also tried to calculate the partial speciﬁc volume of the PEG derivative in aqueous solutions using established methods [27, 28]. 2% higher than the experimental results (the same deviation was observed Table 1 Speciﬁc volumes of the poly(ethylene glycol) derivative in diﬀerent solvents and solvent mixtures, as determined by digital densimetry and the buoyant density method. The density, q0, and the dielectric constant, e, of the solvents, at 20 °C, are taken from Refs. 743 28 with unmodiﬁed PEG ).
1a) was obtained by modifying a commercial precursor (Fig. 3 mg 1-pyrene isothiocyanate (Molecular Probes) in 2 ml DMF was added, and the mixture was stirred for 48 h at 40 °C. After evaporation of the solvent, the compound was redissolved in dichlormethane and puriﬁed by size-exclusion chromatography on Bio Beads SX1 (Bio Rad; 70 · 5 cm) in the same solvent. According to matrix-assisted laser desorption ionization time of ﬂight mass spectroscopy using a Voyager DE Pro apparatus (Perceptive Biosystems) with a-cyano cinnamic acid/tetrahydrofuran (THF) as a matrix, the puriﬁed compound was characterized by Mn ¼ 3,637 g/mol and Mw ¼ 3,679 g/mol.
Analytical Ultracentrifugation VI by W. Mächtle, M. D. Lechner (auth.), W. Borchard, A. Straatmann (eds.)